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Induction of Myogenic Differentiation in Myoblasts by Electrical Stimulation
J Korean Soc Phys Med 2019;14(2):63-70
Published online May 31, 2019;
© 2019 Journal of The Korean Society of Physical Medicine.

Hyeon-Jeong Je, PT, MS · Min-Gu Kim, PT, MS · Il-Hoon Cho, PhD1,2† · Hyuck-Joon Kwon, PhD

Department of Physical Therapy and Rehabilitation, College of Health Science, Eulji University,
1Department of Biomedical Laboratory Science, College of Health Science, Eulji University,
2Program for BK21+ Senior Healthcare, Graduate school, Daejeon, Republic of Korea
Received April 4, 2019; Revised April 9, 2019; Accepted April 22, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
PURPOSE: While electrical stimulation (ES) is known to be a safe and flexible tool in rehabilitation therapy, it has had limited adoption in muscle regeneration. This study was performed to investigate whether ES can induce myogenic differentiation and to clarify the mechanism underlying the effects of ES on myogenic differentiation.
METHODS: This study used rat L6 cell lines as myoblasts for myogenic differentiation. Electric stimulation was applied to the cells using a C-Pace EP culture pacer (IonOptix, Westwood, Ma, USA). The gene expressions of myogenic markers were examined using qPCR and immunochemistry.
RESULTS: Our study showed that ES increased the thickness and length of myotubes during myogenic differentiation. It was found that ES increased the expression of myogenic markers, such as MyoD and Myogenin, and also activated the fusion of the myoblast cells. In addition, ES suppressed the expression of small GTPases, which can explain why ES promotes myogenic differentiation.
CONCLUSION: We found that ES induced myogenic differentiation by suppressing small GTPases, inhibiting cell division. We suggest that ES-based therapies can contribute to the development of safe and efficient muscle regeneration.
Keywords : Electrical stimulations, Myoblast, Myogenesis, Small GTPase

May 2019, 14 (2)
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